Environmental Health
Algal and Other Environmental Toxins Laboratory
About the Laboratory
Scientists at the Organic Geochemistry Research Laboratory (OGRL) in Lawrence, Kansas, develop and employ targeted and non-targeted analytical methods for identification and quantitation of known and understudied algal/cyanobacterial toxins that can impact the health of humans and other organisms. Newly acquired (2018) instrumentation will expand capabilities to continue toxin detection with increased throughput, connect toxin exposure with biomarkers to meet the growing demand for reliable algal toxin data and better define health effects thresholds.
Current Algal/Cyanobacterial Toxins Capabilities
Scientists reviewing data to determine instrument performance at the Algal Toxins Laboratory in Lawrence Kansas.
(Credit: Keith Loftin, USGS. Public domain.)
Cyanotoxins
- anatoxin-a
- BMAA
- cylindrospermopsin
- 10 microcystins
- nodularin-R
- saxitoxins
Marine Toxins
- azaspiracid-1
- domoic acid
- dinophysistoxin-2
- gymnodimine
- okadaic acid
- pectinotoxin-2
- 13-desmethyl-spirolide C
- saxitoxins
Key Instrumentation
- New (2019) 2,500 square foot modern laboratory facility
- Onebioinert liquid chromatography triple quadrupole mass spectrometers for quantitation
- One bioinert liquid chromatography high resolution mass spectrometers for identification of unknown chemicals and biomarkers of exposure
- One tritimeter
- An automated bioassay plate reader for toxin screening and toxicity endpoint measurement
- An infrared spectrophotometer for chemical screening and structure illucidation
- A handheld x-ray fluorescence analyzer for elemental analysis screening (such as metals)
- One handheld hyperspectral radiometer for satellite validation
Keith A. Loftin, USGS, is the lead scientist for algal and cyanobacterial toxins laboratory and the Algal and other Natural Toxins Integrated Science Team (Credit: Ariel Donovan, USGS. Public domain.)
Related Content
Associations between cyanobacteria and indices of secondary production in the western basin of Lake Erie
Cyanobacterial blooms may influence secondary production because 1) cyanobacteria are a poor quality food resource and 2) cyanotoxins may be harmful to consumers. Over three years at 36 sites across the western basin of Lake Erie, we measured 3 indices of secondary production: growth of a native unionid mussel, the size of young-of-year dreissenid mussels, and the mass of animals on an HD...
Microcystin, chlorophyll, and cell-count data for assessing the effect of salinity tolerance on cyanobacteria associated with a harmful algal bloom in Lake Okeechobee, Florida, July 9 to 17, 2017
This U.S. Geological Survey (USGS) Data Release provides microcystin, chlorophyll, and cell-count data for assessing the effect of salinity tolerance on cyanobacteria associated with a harmful algal bloom in Lake Okeechobee, Florida. All data are reported as raw measured values and are not rounded to USGS significant figures. Water and algal bloom material were collected from Lake Okeec
Velocity test data for assessing the effect of salinity tolerance on cyanobacteria associated with a harmful algal bloom in Lake Okeechobee, Florida, July 10 to 13, 2017
This U.S. Geological Survey (USGS) Data Release provides velocity test data for assessing the effect of salinity tolerance on cyanobacteria associated with a harmful algal bloom in Lake Okeechobee, Florida, July 10 to 13, 2017. All data are reported as raw measured values and are not rounded to USGS significant figures. Water and algal bloom material were collected from Lake Okeechobee%
Fourier Transform Infrared (FTIR) screening of extracted sediments for samples collected for the Sediment-Bound Contaminant Resiliency and Response Strategy pilot study, northeastern United States, 2015
Coastal communities are uniquely vulnerable to sea-level rise and severe storms such as hurricanes. These events enhance the dispersion and concentration of natural and anthropogenic chemicals and pathogenic microorganisms that could adversely affect the health and resilience of coastal communities and ecosystems in subsequent years. The U.S. Geological Survey has developed the Sediment-Bound...
Protein Phosphatase 2A screening of extracted sediments for samples collected for the Sediment-Bound Contaminant Resiliency and Response Strategy pilot study, northeastern United States, 2015.
Coastal communities are uniquely vulnerable to sea-level rise and severe storms such as hurricanes. These events enhance the dispersion and concentration of natural and anthropogenic chemicals and pathogenic microorganisms that could adversely affect the health and resilience of coastal communities and ecosystems in subsequent years. The U.S. Geological Survey has developed the Sediment-Bound...
Cyanotoxin, chlorophyll-a, and cyanobacterial toxin genetic data for samples collected at eleven large river sites throughout the United States, June through September 2017
This U.S. Geological Survey (USGS) data release provides discretely measured cyanotoxin, chlorophyll-a, and cyanobacterial toxin genetic data for samples collected from eleven large river sites throughout the United States, from June through September 2017. Discrete water-quality samples were analyzed for cyanotoxins (anatoxin, cylindrospermopsin, microcystin, and saxitoxin)%2
Periphyton (1993-2011) and Water Quality (2014) Data for ET&C Article Entitled Spatial and Temporal Variation in Microcystins Occurrence in Wadeable Streams in the Southeastern USA
Spatial reconnaissance of fluvial microcystins (MC) concentrations and select water-quality parameters, including nutrients and periphyton biomass, in 75 wadeable streams in the Piedmont region of the southeastern USA during 2014. Data set includes only those data specifically discussed in the associated journal article: Loftin, K.A., Clark, J.M., Journey, C.A., Kolpin, D.
Mixed organic and inorganic tapwater exposures and potential effects in greater Chicago area, USA
Safe drinking water at the point of use (tapwater, TW) is a public-health priority. TW exposures and potential human-health concerns of 540 organics and 35 inorganics were assessed in 45 Chicago area United States (US) homes in 2017. No US Environmental Protection Agency (EPA) enforceable Maximum Contaminant Level(s) (MCL) were exceeded in any...
Bradley, Paul; Argos, Maria; Kolpin, Dana W.; Meppelink, Shannon M.; Romanok, Kristin; Smalling, Kelly; Focazio, Michael J.; Allen, Joshua M.; Dietze, Julie E.; Devito, Michael J.; Donovan, Ariel; Evans, Nicola; Givens, Carrie E.; Gray, James L.; Higgins, Christopher P.; Hladik, Michelle; Iwanowicz, Luke; Journey, Celeste A.; Lane, Rachael; Laughrey, Zachary; Loftin, Keith A.; McCleskey, R. Blaine; McDonough, Carrie A.; Medlock Kakaley, Elizabeth K; Meyer, Michael T.; Holthouse-Putz, Andrea; Richardson, Susan D; Stark, Alan ; Weis, Christopher P.; Wilson, Vickie S.; Zehraoui, Abderrahman
Methods used for the collection and analysis of chemical and biological data for the Tapwater Exposure Study, United States, 2016–17
In 2016, the U.S. Geological Survey (USGS) Environmental Health Mission Area, initiated the Tapwater Exposure Study as part of an infrastructure project to assess human exposure to potential threats from complex mixtures of contaminants. In the pilot phase (2016), samples were collected from 11 States throughout the United States, and in the...
Romanok, Kristin M.; Kolpin, Dana W.; Meppelink, Shannon M.; Argos, Maria; Brown, Juliane B.; Devito, Michael J.; Dietze, Julie E.; Givens, Carrie E.; Gray, James L.; Higgins, Christopher P.; Hladik, Michelle L.; Iwanowicz, Luke R.; Loftin, Keith A.; McCleskey, R. Blaine; McDonough, Carrie A.; Meyer, Michael T.; Strynar, Mark J.; Weis, Christopher P.; Wilson, Vickie S.; Bradley, Paul M.
Understanding the effect of salinity tolerance on cyanobacteria associated with a harmful algal bloom in Lake Okeechobee, Florida
In an effort to simulate the survival of cyanobacteria asthey are transported from Lake Okeechobee to the estuarinehabitats that receive waters from the lake, a bioassayencompassing a range of salinities was performed. An overalldecline in cyanobacteria health in salinity treatments greaterthan 18 practical salinity units (psu) was indicated by...
Rosen, Barry H.; Loftin, Keith A.; Graham, Jennifer L.; Stahlhut, Katherine N.; Riley, James M.; Johnston, Brett D.; Senegal, Sarena
Water-quality conditions with an emphasis on cyanobacteria and associated toxins and taste-and-odor compounds in the Kansas River, Kansas, July 2012 through September 2016
Cyanobacteria cause a multitude of water-quality concerns, including the potential to produce toxins and taste-and-odor compounds that may cause substantial economic and public health concerns, and are of particular interest in lakes, reservoirs, and rivers that are used for drinking-water supply. Extensive cyanobacterial blooms typically do not...
Graham, Jennifer L.; Foster, Guy M.; Williams, Thomas J.; Mahoney, Matthew D.; May, Madison R.; Loftin, Keith A. Book review: Handbook of cyanobacterial monitoring and cyanotoxin analysis
Review of Meriluoto, Jussi, Lisa Spoof, and GeoffreyA. Codd [eds.]. 2017. Handbook of Cyanobacterial Monitoring and Cyanotoxin Analysis. John Wiley & Sons, Ltd.: Chichester, West Sussex, UK, ISBN 978‐1‐119‐06868‐6 (978‐1‐119‐06876‐1 eBook), DOI 10.1002/9781119068761.
Graham, Jennifer L.; Loftin, Keith A. Associations between cyanobacteria and indices of secondary production in the western basin of Lake Erie
Large lakes provide a variety of ecological services to surrounding cities and communities. Many of these services are supported by ecological processes that are threatened by the increasing prevalence of cyanobacterial blooms which occur as aquatic ecosystems experience cultural eutrophication. Over the past 10 yr, Lake Erie experienced...
Larson, James H.; Evans, Mary Anne; Kennedy, Robert J.; Bailey, Sean; Loftin, Keith A.; Laughrey, Zachary; Femmer, Robin; Schaeffer, Jeff; Richardson, William B.; Wynne, Timothy; Nelson, J. C.; Duris, Joseph W.
Detection of microcystin and other cyanotoxins in lakes at Isle Royale National Park, Pictured Rocks National Lakeshore, and Sleeping Bear Dunes National Lakeshore, northern Michigan, 2012–13
Although cyanotoxins released during algal blooms have become an increasing concern in surface waters across the United States, the presence of cyanotoxins in northern Michigan lakes had not been evaluated in detail. The U.S. Geological Survey and National Park Service (NPS) led a 2-year study (2012 and 2013) to determine the presence of...
Fuller, Lori M.; Brennan, Angela K.; Fogarty, Lisa R.; Loftin, Keith A.; Johnson, Heather E.; VanderMeulen, David D.; Lafrancois, Brenda Moraska Satellite monitoring of cyanobacterial harmful algal bloom frequency in recreational waters and drinking water sources
Cyanobacterial harmful algal blooms (cyanoHAB) cause extensive problems in lakes worldwide, including human and ecological health risks, anoxia and fish kills, and taste and odor problems. CyanoHABs are a particular concern in both recreational waters and drinking water sources because of their dense biomass and the risk of exposure to toxins....
Clark, John M.; Schaeffer, Blake A.; Darling, John A.; Urquhart, Erin A. ; Johnston, John M.; Ignatius, Amber R.; Myer, Mark H.; Loftin, Keith A.; Werdell, P. Jeremy; Stumpf, Richard P. A method for examining temporal changes in cyanobacterial harmful algal bloom spatial extent using satellite remote sensing
Cyanobacterial harmful algal blooms (CyanoHAB) are thought to be increasing globally over the past few decades, but relatively little quantitative information is available about the spatial extent of blooms. Satellite remote sensing provides a potential technology for identifying cyanoHABs in multiple water bodies and across geo-political...
Urquhart, Erin A. ; Schaeffer, Blake A.; Stumpf, Richard P. ; Loftin, Keith A.; Werdell, P. Jeremy A critical review of the postulated role of the non-essential amino acid, β-N-methylamino-L-alanine, in neurodegenerative disease in humans
The compound BMAA (β-N-methylamino-L-alanine) has been postulated to play a significant role in four serious neurological human diseases: Amyotrophic Lateral Sclerosis/Parkinsonism Dementia Complex (ALS/PDC) found on Guam, and ALS, Parkinsonism, and dementia that occur globally. ALS/PDC with symptoms of all three diseases first came to the...
Chernoff, Neil; Hill, D. J.; Diggs, D. L.; Faison, B. D.; Francis, B. M.; Lang, J. R.; Larue, M. M.; Le, T.-T.; Loftin, Keith A.; Lugo, J. N.; Schmid, J. E.; Winnik, W. W.
Water quality and bed sediment quality in the Albemarle Sound, North Carolina, 2012–14
The Albemarle Sound region was selected in 2012 as one of two demonstration sites in the Nation to test and improve the design of the National Water Quality Monitoring Council’s National Monitoring Network (NMN) for U.S. Coastal Waters and Tributaries. The goal of the NMN for U.S. Coastal Waters and Tributaries is to provide information about the...
Moorman, Michelle C.; Fitzgerald, Sharon A.; Gurley, Laura N.; Rhoni-Aref, Ahmed ; Loftin, Keith A.
Spatial variability of harmful algal blooms in Milford Lake, Kansas, July and August 2015
Cyanobacterial harmful algal blooms (CyanoHABs) tend to be spatially variable vertically in the water column and horizontally across the lake surface because of in-lake and weather-driven processes and can vary by orders of magnitude in concentration across relatively short distances (meters or less). Extreme spatial variability in cyanobacteria...
Foster, Guy M.; Graham, Jennifer L.; Stiles, Tom C.; Boyer, Marvin G.; King, Lindsey R.; Loftin, Keith A.